- Low background due to the elimination of autofluorescence
- Bright luminescence with discrete emission bands
- No photobleaching or self-quenching
- Detectable through tissue and whole blood
- Particle and reader manufacturing is done according to ISO 13485
Our customer was not able to measure clinically relevant concentrations with their current system (latex beads, sensitivity demand <500 pg/ml).
By changing to Upcon® technology, they reached targeted sensitivity with minimal optimization work (dry strips 4 pg/ml or wet strips 50 pg/ml). (Data courtesy of Medix Biochemica, Finland).
Lahtinen, S. (2019) Upconverting Nanoparticles in Bioaffinity Assays: New Insights and Perspectives. http://urn.fi/URN:ISBN:978-951-29-7769-7
Arppe, R. (2015) Photon Upconverting Nanophosphors: Unique Reporters for Optical Biosensing. http://urn.fi/URN:ISBN:978-951-29-6182-5
Riuttamäki, T. (2011) Upconverting Phosphor Technology: Exceptional Photoluminescent Properties Light Up Homogeneous Bioanalytical Assays. http://urn.fi/URN:ISBN 978-951-29-4743-0
Lateral flow tests (immunochromatographic dipsticks)
Sousa , M.S., et al. (2019) Performance of an Ultra-Sensitive Assay Targeting the Circulating Anodic Antigen (CAA) for Detection of Schistosoma mansoni Infection in a Low Endemic Area in Brazil. Front Immunol. 10: 682. doi: 10.3389/fimmu.2019.00682
Juntunen, E. et al. (2017) Lateral Flow Immunoassay With Upconverting Nanoparticle-Based Detection for Indirect Measurement of Interferon Response by the Level of MxA. J. Med. Virol. 89: 598.
doi: 10.1002/jmv.24689 van Hooij, A. et al. (2017) Field-friendly serological tests for determination of M. leprae-specific antibodies.
Sci. Rep. 7: 8868. doi: 10.1038/s41598-017-07803-7
Sedlmeier, A. et al. (2016) Highly Sensitive Laser Scanning of Photon-Upconverting Nanoparticles on a Macroscopic Scale. Anal. Chem. 88: 1835. doi: 10.1021/acs.analchem.5b04147.